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Alterations of surface markers on SW982 cells pretreated after HDACi for 48 h. The MFIs of CD80 ( A , B ), HLA-ABC ( C , D ) and PD-L1 ( E , F ) expression on panobinostat or vorinostat pretreated SW982 cells are displayed. DMSO served as solvent control. Histograms are showing representative flow cytometry results. Data analyzed contain at least four individual experiments with duplicates. Statistical significance was calculated using a paired two-way student t test. * p < 0.05.

Journal: Biomedicines

Article Title: HDAC Inhibition for Optimized Cellular Immunotherapy of NY-ESO-1-Positive Soft Tissue Sarcoma

doi: 10.3390/biomedicines10020373

Figure Lengend Snippet: Alterations of surface markers on SW982 cells pretreated after HDACi for 48 h. The MFIs of CD80 ( A , B ), HLA-ABC ( C , D ) and PD-L1 ( E , F ) expression on panobinostat or vorinostat pretreated SW982 cells are displayed. DMSO served as solvent control. Histograms are showing representative flow cytometry results. Data analyzed contain at least four individual experiments with duplicates. Statistical significance was calculated using a paired two-way student t test. * p < 0.05.

Article Snippet: The following fluorochrome-conjugated antibodies were used for immunophenotyping of different surface markers on SW982 cells as well as NY-ESO-1-specific T cells: anti-CD80-APC (BioLegend, San Diego, CA, USA), anti-HLA-ABC-FITC (eBioscience, San Diego, CA, USA), anti-programmed cell death protein ligand 1 (PD-L1)-PE (eBioscience), anti-CD3-V500 (AmCyan) (BD Biosciences, San Diego, CA, USA), anti-CD8-Parcific blue (BioLegend), anti-CD25-PE-Cy7 (BioLegend), anti-CD4-Alexa Fluor 700 (eBioscience).

Techniques: Expressing, Flow Cytometry